Determining binding affinity (KD) of proteins
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Selected links to publications addressing methods for determining binding affinities.
At present most of these refer to anisotropy methods.
BEST ANISOTROPY MANUAL
(Although this manual is very comprehensive, there is a single error in the anisotropy fitting equation)
Article: A Guide to Simple and Informative Binding Assays
Article: Analysis of protein-ligand interactions by fluorescence polarization
Class: Cy dyes, lab handouts about fluorescence anisotropy
Additional links addressing practical aspects of fluorescence anisotropy including basic methods and error analysis.